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Anti-Trimethyl Histone H3(Lys9), clone CMA308
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REFERENCES
Differentially expressed genes are marked by histone 3 lysine 9 trimethylation in human cancer cells.
Wiencke, J K, et al. (2008) Oncogene, 27: 2412-21 (2008)
The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
Kimura, Hiroshi, et al. (2008) Cell Struct. Funct., 33: 61-73 (2008)
Epigenetic histone H3 lysine 9 methylation in metabolic memory and inflammatory phenotype of vascular smooth muscle cells in diabetes.
Villeneuve, Louisa M, et al. (2008) Proc. Natl. Acad. Sci. U.S.A., 105: 9047-52 (2008)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt WB, ELISA, ICC, IP, Mplex Mouse Purified Monoclonal Antibody
Description:
Anti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308
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Specificity:
This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is >27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.
Molecular Weight:
~17 kDa
Epitope:
Trimethyl Lys9
Immunogen:
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, trimethylated on Lys9, conjugated to KLH.
Modifications:
Methylation
Clone:
CMA308
Isotype:
IgG1κ
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for IP.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
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Control:
HeLa Acid extract.
Quality Assurance:
Western Blot Analysis:
A 0.5 – 2 μg/mL dilution of this antibody detected histone H3 in 10 μg of HeLa acid extract.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
H3.
H3/g
H3/t
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