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Anti-Dimethyl Histone H3 (Lys9), clone CMA307 Antibody
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REFERENCES
The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
Kimura, Hiroshi, et al. (2008) Cell Struct. Funct., 33: 61-73 (2008)
Differential H3K4 methylation identifies developmentally poised hematopoietic genes.
Orford, Keith, et al. (2008) Dev. Cell, 14: 798-809 (2008)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt WB, ELISA, ICC, IP, Mplex, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307
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Specificity:
This antibody recognizes Histone H3 dimethylated at Lys9. Phosphorylation of Ser10 interferes with antibody binding to dimethyl Lys9.
Molecular Weight:
~17 kDa
Epitope:
dimethyl Lys9
Immunogen:
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, dimethylated on Lys9, conjugated to KLH.
Modifications:
Methylation
Clone:
CMA307
Isotype:
IgG1κ
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
Chromatin Immunoprecipitation::
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG, or Anti-dimethyl Histone H3 (Lys9) antibody, clone CMA307 and the Magna ChIP G (Cat. # 17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using ChIP Primers B-Globin.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 05-1249, Anti-dimethyl H3 (Lys9), clone CMA307 at 2.0ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA. Kimura, H., et al. (2008). Cell Struct. Funct. 33: 61-73.
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry ( Kimura, 2008).
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation ( Kimura, 2008).
Multiplexing:
This antibody specifically recognizes histone H3 dimethylated on Lys9 by Luminex® assay.
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Control:
HeLa acid extract
Quality Assurance:
Western Blot Analysis: 1:10,000 of th |