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Anti-Ubiquitin, Lys48-Specific,
clone Apu2
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REFERENCES
RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
Mallette, Frédérick A, et al. (2012) EMBO J., 31: 1865-78 (2012)
CARMA1 controls Th2 cell-specific cytokine expression through regulating JunB and GATA3 transcription factors.
Marzenna Blonska,Donghyun Joo,Patrick A Zweidler-McKay,Qingyu Zhao,Xin Lin (2012) Journal of immunology (Baltimore, Md. : 1950).188
Regulation of Toll-like receptor signaling by NDP52-mediated selective autophagy is normally inactivated by A20.
Megumi Inomata,Shumpei Niida,Ken-Ichiro Shibata,Takeshi Into (2012) Cellular and molecular life sciences : CMLS.69
De-ubiquitinating protease USP2a targets RIP1 and TRAF2 to mediate cell death by TNF.
Mahul-Mellier, A-L, et al. (2011) Cell death and differentiation, (2011)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Rb FC, ICC, IP, IHC, WB, Cell Function Assay Rabbit Purified Monoclonal Antibody
Description:
Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal
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Product Overview:
DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)
Specificity:
Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
Molecular Weight:
varies
Epitope:
Lys48 ubiquitination site
Immunogen:
Immunogen used was human di-ubiquitin linked at Lys48.
Clone:
Apu2
Isotype:
IgG
Background Information:
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
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Species Reactivity:
Human
Mouse
Rat
Rabbit
Species Reactivity Note:
Human, mouse, rat, rabbit. Based on sequence homology, broad species cross-reactivity is expected.
Application Notes:
Immunohistochemistry:
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.
Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).
Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
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Control:
Lys48 polyubiquitin chains.
Quality Assurance:
Western Blot Analysis:
A 1:1000-1:5000 dilution of thi |