|
Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4
Close
REFERENCES
High levels of glucose induce metabolic memory in cardiomyocyte via epigenetic histone H3 lysine 9 methylation.
Xi-Yong Yu,Yong-Jian Geng,Jia-Liang Liang,Saidan Zhang,He-Ping Lei,Shi-Long Zhong,Qiu-Xiong Lin,Zhi-Xin Shan,Shu-Guang Lin,Yangxin Li (2012) Molecular biology reports.39
M-Track: detecting short-lived protein-protein interactions in vivo.
Zuzuarregui, Aurora, et al. (2012) Nat. Methods, 9: 594-6 (2012)
Interplay between oncogene-induced DNA damage response and heterochromatin in senescence and cancer.
Di Micco, Raffaella, et al. (2011) Nat. Cell Biol., 13: 292-302 (2011)
Differentially expressed genes are marked by histone 3 lysine 9 trimethylation in human cancer cells.
Wiencke, J K, et al. (2008) Oncogene, 27: 2412-21 (2008)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M PIA, IF, DB, ChIP-seq, WB, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Recognizes trimethyl-histone H3 (Lys9), Mr 17 kDa. Cross-reacts with dimethyl histone H3(Lys9) at higher concentrations.
Molecular Weight:
~17 kDa
Epitope:
Trimethyl Lys9
Immunogen:
KLH-conjugated, branched synthetic peptide containing the sequence (QTARme3K- STGGKA)2-KC, in which me3K corresponds to trimethyl lysine 9 of human histone H3. Data suggests this antibody may require the presence of the glutamine residue in position 5 for binding.
Modifications:
Methylation
Clone:
6F12-H4
Isotype:
IgG1κ
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α/β. The enzymes responsible for H3K9me3 formation are SUV39H1 and SUV39H2.
View All »
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse. Based on sequence homology, broad species cross-reactivity is expected with all mammals, Drosophila, Xenopus, and Arabidopsis.
Application Notes:
Chromatin Immunoprecipitation (ChIP):
Representative data from a previous lot. Sonicated 3T3 L1 chromatin was subjected to chromatin immunoprecipitation using anti- trimethyl-histone H3 (Lys9) and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of trimethylhistone H3 (Lys9) associated DNA fragments was verified by qPCR using primers flanking the p16 promoter.
Peptide Inhibition Analysis:
Peptide blocking assay demonstrates distinct preference of the antibody for the trimethyl form vs. the dimethyl form.
Chromatin Immunoprecipitation (ChIP):
ChIP analysis of kno |