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Anti-Sirt1 Antibody, clone 3H10.2
Recommended Replacement for: 05-707
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REFERENCES
Sirt1 contributes critically to the redox-dependent fate of neural progenitors.
Prozorovski, Timour, et al. (2008) Nat. Cell Biol., 10: 385-94 (2008)
Sirtuins: novel targets for metabolic disease.
Elliott, Peter J and Jirousek, Michael (2008) Current opinion in investigational drugs (London, England : 2000), 9: 371-8 (2008)
hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
Vaziri, H, et al. (2001) Cell, 107: 149-59 (2001)
p53 Activation: a case against Sir.
Brooks, Christopher L and Gu, Wei (2008) Cancer Cell, 13: 377-8 (2008)
Species Reactivity Key Applications Host Format Antibody Type
H IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Sirt1 Antibody, clone 3H10.2
Replaces:
05-707
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Trade Name:
Upstate (Millipore)
Specificity:
Human Sirt1. Does not cross react with lysates from mouse NIH/3T3 cells or rat PC12 cells.
Molecular Weight:
120 kDa
Immunogen:
GST fusion protein corresponding to amino acids 508-616 of human Sirt1.
Clone:
3H10.2
Isotype:
IgG1
Background Information:
The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan.
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Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on nuclear extract from HeLa cells
Purification Method:
Protein G purfied
Presentation:
Purified in PBS with 0.05% Sodium Azide
Storage Conditions:
Maintain at 2-8 °C in undiluted aliquots for up to 6 months after date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Q96EB6
Entrez Gene Number:
NM_012238
Gene Symbol:
SIRT1
SIR2L1
hSIR2
OTTHUMP00000060745
hSIRT1
SIR2alpha
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and |