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Anti-acetyl Histone H4 (Lys16), clone 4E10.2
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PRODUCT FAMILY INFORMATION
Histone H4 Antibodies and Proteins
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon.
EMD Millipore’s Histone H4 antibody demonstrates specificity against linker histone H4. See below for data and references for EMD Millipore’s Histone H4 antibodies and proteins. All EMD Millipore antibodies and proteins are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB, PIA Mouse Purified Monoclonal Antibody
Description:
Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes histone H3 acetylated on Lys16, Mr 11 kDa.
Molecular Weight:
~11 kDa
Epitope:
Acetyl Lys16
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence (GKGGA[acetyl-K]RHRKC, corresponding to the sequence surrounding acetylated lysine 16 of human histone H4.
Modifications:
Acetylation
Clone:
4E10.2
Isotype:
IgG1κ
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Global loss of acetylation at Lys16 is a common hallmark of human tumor cells, suggesting a role for acetylated Lys16 in preventing cell transformation. Moreover, Lys16 is the only histone mark that has been directly linked to chromatin refolding and maintainence of chromatin structure. The Sir3 protein helps form telomeric heterochromatin by interacting with hypoacetylated histone H4 Lys16 while the MYST-like acetyltransferase Sas2p is required for the acetylation H4-Lys16 in euchromatin.
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Species Reactivity:
Human
Species Reactivity Note:
Based on sequence homology, broad species cross-reactivity is expected.
Control:
Sodium butyrate-treated HeLa cell acid extracts.
Quality Assurance:
Routinely evaluated by Western Blot
Western Blot Analysis:
A 0.1- 1 μg/mL dilution of a recent lot detected acetylated histone H4 (Lys16) in acid extracts of sodium butyrate-treated HeLa cells. The antibody did not detect unmodified recombinant Histone H3 (Catalog # 14-411)
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Purification Method:
Protein G purfied
Presentation:
Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in PBS, 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P62805
Entrez Gene Number:
NP_003529
Alternate Names:
H4K16Ac
Histone H4 (acetyl K16)
HIST1H4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or |