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Anti-Talin, C-terminus, clone TD77
Recommended Replacement for: MAB3264
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REFERENCES
Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.
Andreas Wortmann,Yaowu He,Melinda E Christensen,Mayla Linn,John W Lumley,Pamela M Pollock,Nigel J Waterhouse,John D Hooper (2011) The Journal of biological chemistry.286
Species Reactivity Key Applications Host Format Antibody Type
H, Ch, M WB, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-Talin Antibody, CT, clone TD77
Replaces:
MAB3264
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Specificity:
Recognizes Human Talin, and cross reacts with Mouse and chicken Talin. The epitope recognized by this antibody is located within the C-terminal region of the protein (residues 2269-2541), which also contains an F-actin binding site homologous to that in the yeast actin-binding protein SIa2p.
Molecular Weight:
270 kDa
Epitope:
C-terminus
Immunogen:
Human platelet protein
Clone:
TD77
Isotype:
IgG1
Background Information:
Talin consisting of a 47-kDa N-terminal head domain (residues 1-433) and a 190-kDa
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
Species Reactivity:
Human
Chicken
Mouse
Species Reactivity Note:
Proven to react with human. Expected cross-reactivity in mouse and chicken.
Application Notes:
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Talin in human GI track tissue.
Control:
Jurkat cell lysate.
Quality Assurance:
Evaluated by Western Blot in Jurkat cell lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
Purification Method:
Purified from Protein A Sepharose™ chromatography
Presentation:
Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PBS pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
Storage Conditions:
Maintain between 2 and 8°C for 1 year after date of receipt.
UniProt Number:
Q9Y490
Entrez Gene Number:
Q9Y490
Gene Symbol:
ILWEQ
KIAA1027
TLN
Alternate Names:
talin 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts By similarity.
SUBUNIT STRUCTURE: Binds with high affinity to vinculin and with low affinity to integrins. Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM.
SUBCELLULAR LOCATION: Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with LAYN at the membrane ruffles By similarity.
SEQUENCE SIMILARITIES: Contains 1 FERM domain.
Contains 1 I/LWEQ domain.
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Product Name:
Anti-Tali |