|
Anti-phospho-Paxillin (Tyr31), clone M102
Recommended Replacement for: MAB1146
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Paxillin (Tyr31) Antibody, clone M102
Replaces:
MAB1146
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
The antibody detects Paxillin phosphorylated at Tyr31.
Molecular Weight:
72 kDa
Immunogen:
Clone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
Modifications:
Phosphorylation
Clone:
M102
Isotype:
IgG1
Background Information:
Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human.
Control:
Pervanadate treated L6 cell lysates
Quality Assurance:
Evaluated by western Blot in pervanadate treated L6 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates.
Purification Method:
Protein A chromatography
Presentation:
Purified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
Storage Conditions:
Maintain at -20°C for one year from date of receipt. Do not aliquot.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
P49023
Entrez Gene Number:
P49023
Gene Symbol:
FLJ16691
paxillin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
SUBUNIT STRUCTURE: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 By similarity. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion.
PTM: Pho |