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Anti-phospho-Focal Adhesion Kinase (Tyr397), clone 18
Recommended Replacement for: MAB1144
Species Reactivity Key Applications Host Format Antibody Type
H, M WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18
Replaces:
MAB1144
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Specificity:
Reacts specifically with Focal Adhesion Kinase (FAK) when phosphorylated on Tyr397. FAK is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr577) in the kinase domain activation loop.
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Molecular Weight:
125 kDa
Epitope:
Tyrosine 397
Immunogen:
Generated from human FAK, a.a. 393-404, phosphorylated on Tyr397.
Modifications:
Phosphorylation
Clone:
18
Isotype:
IgG1
Background Information:
FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. Tyrosine 397 is the autophosphorylation site of Focal Adhesion Kinase. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Proven to react with human and mouse.
Control:
LPS treated RAW 264 lysates.
Quality Assurance:
Evaluated by Western Blot in LPS treated RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-FAK (Tyr397) on 10 ug of LPS treated RAW 264 lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA, and < 0.09% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Q05397
Entrez Gene Number:
Q05397
Gene Symbol:
EC 2.7.10.2
FADK
FAK
FAK1
pp125FAK
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Alternate Names:
FADK 1
PTK2 protein tyrosine kinase 2
Protein-tyrosine kinase 2
focal adhesion kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to e |