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Anti-Focal Adhesion Kinase, clone BLAb2H7
Recommended Replacement for: MAB2156
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ch WB, IP Mouse Purified Monoclonal Antibody
Description:
Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7
Replaces:
MAB2156
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Specificity:
This antibody recognizes Focal Adhesion Kinase
Molecular Weight:
~125 kDa
Epitope:
Terminal
Immunogen:
Recombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553.
Clone:
BLAb2H7
Isotype:
IgG1
Background Information:
FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al).
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Species Reactivity:
Human
Mouse
Rat
Chicken
Species Reactivity Note:
Demonstrated to react with human, mouse, rat and chicken.
Application Notes:
Immunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue.
Control:
HEK293 cell lysate
Quality Assurance:
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q05397
Entrez Gene Number:
NP_990766.1
Gene Symbol:
EC 2.7.10.2
FADK
FAK
FAK1
pp125FAK
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Alternate Names:
FADK 1
PTK2 protein tyrosine kinase 2
Protein-tyrosine kinase 2
focal adhesion kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
UniProt Summary:
FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysop |