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Anti-PDGFR Antibody, clone CH-3
Recommended Replacement for: 05-1135 is a recommended replacement for MAB3091
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ELISA, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-PDGFR Antibody, clone CH-3
Replaces:
05-1135 is a recommended replacement for MAB3091
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Molecular Weight:
211 amino acids; 24043 Da
Immunogen:
Recombinant Platelet-Derived Growth Factor Receptor
Clone:
CH-3
Isotype:
IgG1
Background Information:
PDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and mouse. Expected to react with rat based on sequence homology.
Application Notes:
ELISA: A previous lot of this antibody was used in ELISA.
Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of PDGF-A With Citrate pH 6.0 Epitope Retrieval: Rat Brain
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Control:
Jurkat cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PDGFR on 10 μg of Jurkat lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 20 mM sodium phosphate, 250 mM NaCl, 0.1% NaN3, pH. 7.6
Storage Conditions:
Maintain at + 2-8 ° C in undiluted aliquots for up to 1 year from date of receipt.
UniProt Number:
P09619
Alternate Names:
Platelet Derived Growth Factor Receptor Beta
PDGF-R-beta
PDGFR-beta
Beta platelet-derived growth factor receptor
Beta-type platelet-derived growth factor receptor
CD140 antigen-like family member B
Platelet-derived growth factor receptor 1
PDGFR-1
CD140b
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
ELISA
Immunohistochemistry (Paraffin)
Product Name:
Anti-PDGFR Antibody, clone CH-3
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Applications:
Th |