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Anti-erbB4 Antibody, clone HFR1/2G4
Recommended Replacement for: 05-1133 is a recommended replacement for MAB4025
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-erbB4 Antibody, clone HFR1/2G4
Replaces:
05-1133 is a recommended replacement for MAB4025
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Specificity:
Reactive with human c-erB-4/Her 4 cytoplasmic domain of the protein (residues 1250-1264), a member of the EGF receptor (type 1) family involved in cell growth regulation. Expression or function may be altered in human cancers.
Molecular Weight:
~147 kDa
Epitope:
Cytoplasmic domain of the protein, residues 1250-1264.
Immunogen:
Synthetic peptide sequence RSTLQHPDYLQEYST from the cytoplasmic domain of the protein, residues 1250-1264.
Clone:
HFR1/2G4
Isotype:
IgG1κ
Background Information:
v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and Rat. Expected to react with mouse based on sequence homology.
Application Notes:
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: Effective for formalin-fixed, paraffin-embedded sections.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of erbB-4/HER-4 Monoclonal Antibody: DCIS and Pancreatic Cancer
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Control:
A431 cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected C-ERB-4 on 10 μg of A431 lysates.
Purification Method:
Purified
Presentation:
Purified mouse monoclonal IgM in 0.02 M PB with 0.25 M NaCl, pH 7.6, 0.01% Sodium Azide.
Storage Conditions:
Stable refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
UniProt Number:
Q15303
Entrez Gene Number:
NP_005226.1
Gene Symbol:
ERBB4
c-erbB-4
c-erbB4
p180erbB4
erbB-4
MGC138404
HER4
EC 2.7.10
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
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