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Anti-erbB-3 Antibody, clone RTJ1/2E11
Recommended Replacement for: 05-1131 is a recommended replacement for MAB4021
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IH(P), IP Mouse Ascites Monoclonal Antibody
Description:
Anti-erbB-3 Antibody, clone RTJ1/2E11
Replaces:
05-1131 is a recommended replacement for MAB4021
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Specificity:
Reactive with the intracellular domain of human c-erB-3.
Molecular Weight:
~148 kDa
Immunogen:
Peptide from the carboxy terminus of human c-erB-3
Clone:
RTJ1/2E11
Isotype:
IgM
Background Information:
erbB3 (HER3, c-erbB3) is a receptor tyrosine kinase (RTK). erbB3 is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. The ErbB family of receptors are a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Though erbB3 is membrane bound and can bind its ligand, it does not have an active kinase domain so it can not transmit this signal without first forming a heterodimer with other EGFR family members. This heterodimerization then leads to proliferation and differentiation. erbB3 is also known to bind Phosphoinositide 3-kinase (PI3 Kinase, PI3K) though six tyrosine phosphorylation sites that PI3K can bind to, which results in the activation of Akt/PKB. erbB3 is known to be amplified in numerous cancers including breast and prostate.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human. Expected to react with mouse and rat based on sequence homology.
Application Notes:
Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of erbB-3/HER-3 Monoclonal Antibody: Pancreatic Cancer
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Control:
Huvec cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected C-ERBB-3 on 10 μg of Huvec lysates.
Presentation:
Purified mouse monclonal IgM ascites, no preservative.
Storage Conditions:
Stable frozen at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
P21860
Entrez Gene Number:
NM_001005915.1
Gene Symbol:
ERBB3
c-erbB-3
LCCS2
c-erbB3
p85-sErbB3
p45-sErbB3
ErbB-3
erbB3-S
erbB-3
MGC88033
HER3
p180-ErbB3
MDA-BF-1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Immunoprecipitation
Entrez Gene Summary:
This gene enc |