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Anti-IGF-IR Antibody, β-subunit, clone 1-2
Recommended Replacement for: 05-1106 is a recommended replacement for MAB1123
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Species Reactivity Key Applications Host Format Antibody Type
H, R, M WB, IH(P) Mouse Affinity Purified Monoclonal Antibody
Description:
Anti-IGF-IR Antibody, β-subunit, clone 1-2
Replaces:
05-1106 is a recommended replacement for MAB1123
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Specificity:
Recognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.
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Molecular Weight:
Recognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)
Epitope:
Between a.a. 1323-1337 of IGF-1R
Immunogen:
A 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.
Clone:
1-2
Isotype:
IgG2b
Background Information:
IGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.
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Species Reactivity:
Human
Rat
Mouse
Species Reactivity Note:
Mouse and rat. Expected to react with human based on sequence homology.
Application Notes:
Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of IGF Monoclonal Antibody: Placenta
Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.
Optimal working dilutions must be determined by end user.
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Control:
C2C12 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.
Purification Method:
Affinity Purfied
Presentation:
Purified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Storage Conditions:
Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
UniProt Number:
P08069
Entrez Gene Number:
NM_000875.3
Gene Symbol:
IGF1R
MGC142170
MGC18216
IGFIR
JTK13
MGC142172
CD221
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any oth |