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Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3
Recommended Replacement for: 05-1104 is a recommended replacement for MAB1139
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REFERENCES
Template to improve glycemic control without reducing adiposity or dietary fat.
(2011) American journal of physiology. Endocrinology and metabolism.300:E779-89
Species Reactivity Key Applications Host Format Antibody Type
H, Mk, M, R ELISA, EA, WB, IH(P), IHC Mouse Purified Monoclonal Antibody
Description:
Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3
Replaces:
05-1104 is a recommended replacement for MAB1139
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Specificity:
Recognizes a protein of 95 kDa, identified as the β-subunit of insulin receptor (IR). It is directed against the C-terminal region of β-subunit and shows no cross-reaction with IGF-receptors.
Molecular Weight:
Recognizes a protein of 95 kDa, identified as the β-subunit of insulin receptor (IR).
Epitope:
100 C-Terminal amino acids of human insulin receptor, β-subunit.
Immunogen:
Recombinant-fragment including the C-terminal 100 amino acids of human insulin receptor, β subunit.
Clone:
CT-3
Isotype:
IgG1
Background Information:
The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.
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Species Reactivity:
Human
Monkey
Mouse
Rat
Species Reactivity Note:
Human, Monkey, Mouse and Rat.
Application Notes:
Immunohistochemistry (frozen and formalin/paraffin):
2-4ug/mL of a previous lot of this antibody was used in immunohistochemistry. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min.
Optimal working dilutions must be determined by end user.
ELISA: A previous lot of this antibody showed to be suitable as a capture antibody for ELISA.
Kinase Assay: A previous lot of this antibody was used for antibody-mediated capture on microplates.
Western Blot: A previous lot of this antibody was used at 1 ug/mL, for 2 hrs at room temperature.
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Control:
NIH/3T3 cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Insulin Receptor, beta subunit on 10 μg of NIH/3T3 lysates.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Storage Conditions:
Stable for 1 year at |