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Anti-STAT3, clone 18F7.1
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-STAT3 Antibody, clone 18F7.1
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Molecular Weight:
~88 kDa observed
Epitope:
Unknown
Immunogen:
KLH-conjugated linear peptide corresponding to human STAT3.
Clone:
18F7.1
Isotype:
IgG1κ
Background Information:
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with human and mouse.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STAT3 in HeLa and A431 cells.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 in 10 µg of HeLa cell lysate.
Purification Method:
Protein G Purified
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P40763
Entrez Gene Number:
NP_003141
Gene Symbol:
APRF
FLJ20882
HIES
MGC16063
Alternate Names:
Acute-phase response factor
DNA-binding protein APRF
signal transducer and activator of transcription 3
signal transducer and activator of transcription 3
(acute-phase response factor)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the a |