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Anti-IRS1 Antibody, clone 4.2.2
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REFERENCES
Rapamycin has a biphasic effect on insulin sensitivity in C2C12 myotubes due to sequential disruption of mTORC1 and mTORC2.
Lan Ye,Behzad Varamini,Dudley W Lamming,David M Sabatini,Joseph A Baur (2012) Frontiers in genetics.3
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Mk, B, Ca, Po WB, IP, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-IRS1 Antibody, clone 4.2.2
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Specificity:
This antibody recognizes IRS1.
Molecular Weight:
~185 kDa
Epitope:
Internal
Immunogen:
Synthetic peptide corresponding to amino acids 431-446 of mouse IRS1.
Clone:
4.2.2
Isotype:
IgG2aκ
Background Information:
IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.
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Species Reactivity:
Human
Mouse
Rat
Monkey
Bovine
Canine
Pig
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Species Reactivity Note:
Human, Mouse, and Rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Application Notes:
Immunocytochemistry:
IR/IRS transfected CHO cells were stained with a 1:500 dilution of the antibody. A seconday anti-mouse Cy3 conjugate was used for visualizaion.
Immunoprecipition:
This antibody was used at a 1:1,000 to immunoprecipitate IRS1 from 5L/mL IR/IRS1 transfected CHO cells (performed by an independent lab).
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Quality Assurance:
Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4),150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
P35568
Entrez Gene Number:
NP_005535.1
Gene Symbol:
HIRS-1
IRS-1
Alternate Names:
insulin receptor substrate 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunocytochemistry
UniProt Summary:
FUNCTION: May mediate the control of various cellular processes by |