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Anti-c-Jun, clone 6E4.4
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-c-Jun Antibody, clone 6E4.4
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Specificity:
This antibody detects c-Jun, regardless of phosphorylation state.
Molecular Weight:
~36-43 kDa.
Multiple bands may be visible, indicating various phosphorylation states.
Immunogen:
GST-tagged recombinant protein corresponding to c-Jun.
Clone:
6E4.4
Isotype:
IgG1κ
Background Information:
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with human, mouse and rat.
Application Notes:
Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).
Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).
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Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of receipt.
UniProt Number:
P45983
Entrez Gene Number:
NP_002219.1
Gene Symbol:
EC 2.7.11.24
JNK
JNK-46
JNK1
JNK1A2
JNK21B1/2
OTTHUMP00000019552
OTTHUMP00000019555
OTTHUMP00000019556
OTTHUMP00000019558
PRKM8
SAPK1
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Alternate Names:
JNK1 alpha protein kinase
JNK1 beta protein kinase
JUN N-terminal kinase
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
mitogen-activated protein kinase 8
mitogen-activated protein kinase 8 isoform JNK1 alpha1
mitogen-activated protein kinase 8 isoform JNK1 beta2
protein kinase JNK1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
This gene is the putative transforming gene of avian sarcoma vir |