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Anti-FOXO1, clone 2H8.2
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-FOXO1 Antibody, clone 2H8.2
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Specificity:
This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.
Molecular Weight:
~78-82 kDa
Immunogen:
GST-tagged recombinant protein corresponding to full length FOXO1.
Clone:
2H8.2
Isotype:
IgG1κ
Background Information:
Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse and rat.
Application Notes:
Immunocytochemistry: A 1:500 dilution of a previous lot was used.
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.
Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
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Control:
HEK293 cell lysate
Quality Assurance:
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q12778
Entrez Gene Number:
NP_002006.2
Gene Symbol:
FKH1
FKHR
FOXO1A
OTTHUMP00000018301
Alternate Names:
Forkhead box protein O1A
Forkhead in rhabdomyosarcoma
forkhead box O1
forkhead box O1A (rhabdomyosarcoma)
forkhead homolog in rhabdomyosarcoma
forkhead, Drosophila, homolog of, in rhabdomyosarcoma
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].
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UniProt Summary:
FUNCTION: Transcription factor.
SUBUNIT STRUCTURE: Inte |