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Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
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Specificity:
Detects PRAS40 when phosphorylated on Thr246.
Molecular Weight:
40 kDa
Epitope:
Phosphorylated Thr246
Immunogen:
Synthetic peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40.
Modifications:
Phosphorylation
Clone:
4H2.2
Isotype:
IgG1κ
Background Information:
PRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and Mouse. Predicted to cross-react with rat and bovine based sequence homology of immunizing peptide.
Quality Assurance:
Evaluated by western blot on untreated and PDGF-treated NIH/3T3 cell lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect PRAS40 in PDGF-treated NIH/3T3 cell lysate.
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Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q96B36
Entrez Gene Number:
NP_001092102.1
Gene Symbol:
PRAS40
AKT1S1
Lobe
Alternate Names:
40 kDa proline-rich AKT substrate
AKT1 substrate 1 (proline-rich)
proline-rich Akt substrate, 40 kDa
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunocytochemistry
Entrez Gene Summary:
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]
UniProt Summary:
FUNCTION: May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SUBUNIT STRUCTURE: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm - cytosol. Not |