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Anti-PRAS40, clone 9D10.2
Species Reactivity Key Applications Host Format Antibody Type
M, H, R WB, IP, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-PRAS40 Antibody, clone 9D10.2
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Specificity:
This antibody recognizes PRAS40.
Molecular Weight:
~ 40 kDa
Epitope:
N/A
Immunogen:
Recombinant protein corresponding to human PRAS40.
Clone:
9D10.2
Isotype:
IgG2bκ
Background Information:
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate, as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to, and regulate mTORC1 (mTOR, Raptor, mLST8) kinase activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it to bind Raptor of mTORC1 in the absence of insulin1. It is thought that PRAS40 inhibits the kinase activity of mTORC1, when bound to Raptor, by preventing binding to its substrates such as p70S6K and 4EBP1.
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Species Reactivity:
Mouse
Human
Rat
Species Reactivity Note:
Demonstrated to react with human, mouse, and rat.
Application Notes:
Western Blot Analysis: 1 µg/mL from a previous lot detected PRAS40 on 10 µg of HeLa and PC12 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated PRAS40 from 100 µg of NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected PRAS40 in NIH/3T3 cells.
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Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected PRAS40 on 10 µg of NIH/3T3 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q96B36
Entrez Gene Number:
NP_001092102.1
Gene Symbol:
AKT1S1
Lobe
PRAS40
Alternate Names:
40 kDa proline-rich AKT substrate
AKT1 substrate 1 (proline-rich)
proline-rich Akt substrate, 40 kDa
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunocytochemistry
Entrez Gene Summary:
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].
UniProt Summary:
FUNCTION: May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress |