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Anti-Insulin Antibody, clone E11D7
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REFERENCES
Activation of PPARδ up-regulates the expression of insulin gene transcription factor MafA and ameliorates glucose-induced insulin secretion impaired by palmitate.
Mingming Cao,Yang Long,Yuzhen Tong,Jun Wan,Nanwei Tong (2012) Molecular and cellular biochemistry.366
Species Reactivity Key Applications Host Format Antibody Type
H, M, R ELISA, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-Insulin Antibody, clone E11D7
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Specificity:
Recognizes insulin
Molecular Weight:
6 kDa
Epitope:
Insulin
Immunogen:
Human recombinant Insulin
Clone:
E11D7
Isotype:
IgG1κ
Background Information:
Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology.
Application Notes:
ELISA (Direct) Analysis:
Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
Quality Assurance:
Immunohistochemistry Analysis:
20 μg/mL of this antibody detected insulin in β cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monolconal IgG1κ in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
P01308
Entrez Gene Number:
NM_000207.2
Gene Symbol:
INS
proinsulin
insulin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
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Product Name:
Anti-Insulin Antibody, clone E11D7
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Applications:
Anti-Insu |