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Anti-Phospho-Stat1 (Tyr701), clone 5C9.2
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REFERENCES
STATs and gene regulation.
Darnell, J E (1997) Science, 277: 1630-5 (1997)
Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha.
Schindler, C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A., 89: 7836-9 (1992)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2
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Specificity:
This antibody recognizes phosphorylated STAT1 at tyrosine 701.
Molecular Weight:
88-92 kDa
Epitope:
Phosphorylated Tyr701
Immunogen:
KLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.
Modifications:
Phosphorylation
Clone:
5C9.2
Isotype:
IgG1κ
Background Information:
STATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Tested on human, mouse and rat.
Control:
EGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.
Quality Assurance:
Routinely evaluated by western blot on EGF treated and untreated A431 cell lysate.
estern Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.
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Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing PBS and 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
P42224
Entrez Gene Number:
NP_009330
Gene Symbol:
ISGF-3
STAT-1
Alternate Names:
Transcription factor ISGF-3 components p91/p84
signal transducer and activator of transcription 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activa |