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Anti-EGFR Antibody, clone LA22
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REFERENCES
Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA (2010) Assay Drug Dev Technol.8:27-36.
RNA interference screen identifies Usp18 as a regulator of epidermal growth factor receptor synthesis.
Jason E Duex,Alexander Sorkin (2009) Molecular biology of the cell.20
Aging-related attenuation of EGF receptor signaling is mediated in part by increased protein tyrosine phosphatase activity.
Kien T Tran, S Diana Rusu, Latha Satish, Alan Wells (2003) Experimental cell research.289:359-67
Ligand-induced lysosomal epidermal growth factor receptor (EGFR) degradation is preceded by proteasome-dependent EGFR de-ubiquitination.
Alwan, Husam A J, et al. (2003) J. Biol. Chem., 278: 35781-90 (2003)
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Species Reactivity Key Applications Host Format Antibody Type
H ICC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-EGFR Antibody, clone LA22
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).
Molecular Weight:
180 kDa
Immunogen:
A431 membrane proteins
Clone:
LA22
Isotype:
IgG2a
Background Information:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
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Species Reactivity:
Human
Species Reactivity Note:
Human.
Application Notes:
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated EGF receptor from 500 μg of A431 RIPA lysate.
Immunocytochemistry:
5 μg/mL of previous lots of the antibody showed positive immunostaining for EGF receptor in A431 cells fixed with 4% paraformaldehyde followed by 100% methanol.
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Control:
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
Evaluatd by RIPA lysates from human A431 cells under reducing conditions.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected EGF receptor in RIPA lysates from human A431 cells under reducing conditions.
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Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2a in storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Frozen solution.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
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