ANTI-SIN1,CLONE 1C7.2

Anti-Sin1, clone 1C7.2
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REFERENCES
Isolation of the mTOR complexes by affinity purification.
Dos D Sarbassov,Olga Bulgakova,Rakhmet I Bersimbaev,Tattym Shaiken (2012) Methods in molecular biology (Clifton, N.J.).821
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IP, IH(P), IF Mouse Purified Monoclonal Antibody
Description:
Anti-Sin1 Antibody, clone 1C7.2
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Specificity:
Detects Sin-1 and its isoforms.
Molecular Weight:
Sin1 is 59 kDa. Isoforms are 36, 37, 41, 53, 54, and 55 kDa.
Immunogen:
Full-length Human Sin1 GST-fusion protein.
Clone:
1C7.2
Isotype:
IgG2aκ
Background Information:
Sin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK.
Sin1 is known to have multiple alternative splicing isoforms. Full-length Sin1 is a 522aa protein (59kDa). Sin1 (Isoform 5) (36 kDa) has exon 6 spliced to an alternative exon 7a that results in the loss of both the RBD and PHL domains. Sin1Isoform 2) (55 kDa) lacks exon 7 and lacks the RBD. Sin1 (Isoform 3) (53 kDa) lacks exon 8 lacks the PHL domain. Sin1 (Isoform 4) (37 kDa) lacks exon 1.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, and Rat. Other species have not been tested.
Quality Assurance:
Western Blot Analysis:
This lot detected Sin1 at 1:1,000 dilution in A431 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
Q9BPZ7
Entrez Gene Number:
NP_001006618
Gene Symbol:
JC310
MGC2745
MIP1
SIN1
Alternate Names:
MEKK2-interacting protein 1
Mitogen-activated protein kinase 2-associated protein 1
SAPK-interacting protein 1
Stress-activated map kinase-interacting protein 1
TORC2 subunit MAPKAP1
mitogen-activated protein kinase associated protein 1
ras inhibitor MGC2745
stress-activated map kinase interacting protein 1
stress-activated protein kinase-interacting 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunohistochemistry (Paraffin)
Immunofluorescence
Entrez Gene Su