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Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2
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REFERENCES
MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation.
Manke, Isaac A, et al. (2005) Mol. Cell, 17: 37-48 (2005)
Crystal structure of the p38 alpha-MAPKAP kinase 2 heterodimer.
ter Haar, Ernst, et al. (2007) J. Biol. Chem., 282: 9733-9 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IF, ELISA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2
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Trade Name:
Upstate (Millipore)
Specificity:
Detects MAPKAP Kinase 2 only when phosphorylated on Thr334.
Molecular Weight:
~50 kDa
Epitope:
Thr334
Immunogen:
KLH-conjugated synthetic peptide containing the sequence and surrounding amino acids phosphorylated Thr334 of human, mouse, rat, bovine and chicken MAPKAP Kinase 2. Immunizing peptide shared 66% homology with phospho-MAPKAP-K3 at Thr313.
Modifications:
Phosphorylation
Clone:
9F10.2
Isotype:
IgG1κ
Background Information:
MAPKAP Kinases are Ser/Thr kinases activated by phosphorylation by the SAPK2/3, or p38 family of stress-activated kinases. MAPKAP Kinase 2 and 3 phosphorylate substrates with the sequence H-X-R-X-X-S where H is any hydrophobic residue, including CREB (Ser 133) and HSP27. MAPKAP kinases are similar to Rsk kinases, which are activated by mitogen-signaling pathways, rather than stress-signaling pathways. MAPKAP Kinase 2 is involved in many cellular processes that include inflammation, stress, and prolifertion.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, Rat. Other species have not been tested.
Application Notes:
Immunofluorescence Confocal Microscopy Analysis:
NIH/3T3 cells were fixed, permeabilized, and stained using Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (Red). Cells were co-stained for actin and nuclear staining with Phalloidin, AlexaFluor® 488 conjugate (Green) and DAPI (Blue), respectively
Control:
HeLa nuclear extract
Quality Assurance:
Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected MAPKAPK2 when phosphorylated on Thr334 at 1:1,000 dilution in PC12 lysates resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
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Purification Method:
Protein G purfied
Presentation:
Protein G purified mouse monoclonal in PBS with 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
UniProt Number:
P49137
Entrez Gene Number:
NP_116584
Gene Symbol:
MAPKAPK-2
Alternate Names:
MAPK-activated protein kinase 2
MAPKAP kinase 2
Mitogen-activated protein kinase-activated protein kinase 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunofluorescence
ELISA
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