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Anti-Phosphoserine, clone 4A4
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REFERENCES
Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.
Qing Lu,Mark S Wainwright,Valerie A Harris,Saurabh Aggarwal,Yali Hou,Thomas Rau,David J Poulsen,Stephen M Black (2012) Free radical biology & medicine.53
Glycine receptor internalization by protein kinases activation.
Miguel Ángel Velázquez-Flores,Rocío Salceda,Miguel Ãngel Velázquez-Flores,RocÃo Salceda (2011) Synapse (New York, N.Y.).65
CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression.
Kong, Xiangfei, et al. (2009) J. Biochem., 146: 417-27 (2009)
Acyl coenzyme A-binding protein (ACBP) is phosphorylated and secreted by retinal Müller astrocytes following protein kinase C activation.
Qian, Zuyuan, et al. (2008) J. Neurochem., 105: 1287-99 (2008)
Species Reactivity Key Applications Host Format Antibody Type
Vrt ELISA, FC, IF, IH(P), WB Mouse Purified Monoclonal Antibody
Description:
Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes serine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
Immunogen:
Phosphoserine coupled to KLH.
Modifications:
Phosphorylation
Clone:
4A4
Isotype:
IgG1
Background Information:
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
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Species Reactivity:
Vertebrates
Species Reactivity Note:
Reacts with vertebrates
Application Notes:
Immunofluorescence
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA
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Control:
Included Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is provided as a free positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC.
Quality Assurance:
Routinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
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Purification Method:
Protein G-Sepharose™ chromatography
Presentation:
Purified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Usage Statement:
Unless otherwise state |