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Anti-Phosphoserine, clone 4A4
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PRODUCT FAMILY INFORMATION
Phosphoserine Antibodies
Millipore’s phosphoserine antibodies, including the popular 4A4 clone, demonstrate sensitivity and detect multiple serine phosphorylations on numerous substrates. See below for pSer antibodies.
Millipore’s phosphoserine antibody demonstrates specificity against Phosphoserine. See below for data and references for Millipore’s phosphoserine antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Phosphoserine/Threonine Antibodies & Kits
Approximately 400 of the 518 kinases in the human genome are serine/threonine specific protein kinases. Millipore offers antibodies for your phospho-Ser/Thr research needs, such as those below.
Approximately 400 of the 518 kinases in the human genome are serine/threonine specific protein kinases and approximately 40 serine/threonine specific protein phosphatases are encoded by the human genome.
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Species Reactivity Key Applications Host Format Antibody Type
Vrt WB, IF, FC, IH(P), ELISA Mouse Purified Monoclonal Antibody
Description:
Anti-Phosphoserine Antibody, clone 4A4
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Trade Name:
Upstate (Millipore)
Specificity:
Serine-phosphorylated proteins from all species
Molecular Weight:
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
Immunogen:
Phosphoserine coupled to KLH
Modifications:
Phosphorylation
Clone:
4A4
Isotype:
IgG1
Background Information:
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
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Species Reactivity:
Vertebrates
Control:
Suggested Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is recommended as a positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20ºC. The lysate is stable for 6 months at –20ºC.
Quality Assurance:
Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Purification Method:
Protein G-Sepharose™ chromatography
Presentation:
1 mL of PBS
Storage Conditions:
2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Key Applications:
Western Blotting
Immunofluorescence
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA
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Brand Family:
Upstate
Product Name:
Anti-Phosphoserine, clone 4A4
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 mg
Format:
Purified
Host:
Mouse
Applications:
This Anti-Phosphoserine Antibody, clone 4A4 is validated for use in WB, IF, FC, IH(P), ELISA for th |