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Anti-CD39, clone 5F2
Species Reactivity Key Applications Host Format Antibody Type
M FC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-CD39 Antibody, clone 5F2
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Specificity:
This antibody recognizes CD39.
Molecular Weight:
~ 100 kDa.
It has been published that CD39 shows molecular weights between ~70-100 kDa (Wang, T.F. et al., 1996).
Epitope:
Unknown
Immunogen:
Recombinant protein corresponding to mouse CD39.
Clone:
5F2
Isotype:
IgG1κ
Background Information:
Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.
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Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with mouse.
Application Notes:
Western Blot Analysis: A previous lot was used by an independent laboratory in WB.
Control:
BALB/c splenocytes
Quality Assurance:
Evaluated by Flow Cytometry in BALB/c splenocytes.
Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P55772
Entrez Gene Number:
NP_033978
Gene Symbol:
CD39
Ecto-apyrase
Ectoapyrase
Alternate Names:
ectonucleoside triphosphate diphosphohydrolase 1
ATPDase
ecto-apyrase
NTPDase-1
Ecto-ATP diphosphohydrolase
Lymphoid cell activation antigen
NTPDase 1
Ecto-apyrase
CD39 antigen
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Western Blotting
UniProt Summary:
FUNCTION: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
CATALYTIC ACTIVITY: ATP + 2 H2O = AMP + 2 phosphate.
COFACTOR: Calcium (By similarity).
MAGNESIUM: (By similarity).
SUBUNIT STRUCTURE: Homodimer; disulfide-linked (By sim |