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Anti-phospho-FAK (Tyr397), clone EP2160Y, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, R WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal
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Specificity:
This antibody detects FAK phosphorylated at Tyr397.
Molecular Weight:
~ 125 kDa
Epitope:
Tyr397
Immunogen:
Phospho-specific peptide surrounding tyrosine 397 of human FAK.
Modifications:
Phosphorylation
Clone:
EP2160Y
Isotype:
IgG
Background Information:
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human and rat.
Control:
Human and rat brain lysates
Quality Assurance:
Evaluated by Western Blot on Human and rat brain cell lysates.
Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect FAK in human and rat brain cell lysates.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q05397
Entrez Gene Number:
Q05397
Gene Symbol:
EC 2.7.10.2
FADK
FAK
FAK1
pp125FAK
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Alternate Names:
FADK 1
PTK2 protein tyrosine kinase 2
Protein-tyrosine kinase 2
focal adhesion kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, prolife |