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Anti-Sonic Hedgehog (C-product) Antibody, clone EP1190Y
Species Reactivity Key Applications Host Format Antibody Type
H WB, IH(P), FC, IP Rabbit null Monoclonal Antibody
Description:
Anti-Sonic Hedgehog (C-product) Antibody, clone EP1190Y, Rabbit | 04-971
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Specificity:
This antibody is specific for both full length (50 kDa) and c-product subunit (27 kDa) of human SHH protein. Sonic hedgehog protein C-product is secreted and can be found in the extracellular space.
Molecular Weight:
50 kDa & 27 kDa
Epitope:
C-product
Immunogen:
A synthetic peptide corresponding to residues within c-product subunit of human SHH protein was used as an immunogen.
Clone:
EP1190Y
Isotype:
IgG
Background Information:
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
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Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation: A 1:70 dilution of a previous lot was used in immunoprecipitation.
Immunohistochemistry (paraffin): A 1:100 dilution of a previous lot was used in immunohistochemsitry.
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Control:
Fetal liver membrane, human kidney tissue.
Quality Assurance:
Evaluated by Western Blotting on fetal liver membrane lysate.
Western Blotting Analysis: 1:2,000 dilution of this antibody detected full length and c-product subunit of sonic hedgehog in fetal liver membrane lysate.
Presentation:
Rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
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UniProt Number:
Q15465
Entrez Gene Number:
NP_990152.1
Gene Symbol:
HHG-1
HHG1
HLP3
HPE3
MCOPCB5
SMMCI
TPT
TPTPS
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Alternate Names:
sonic hedgehog homolog (Drosophila)
sonic hedgehog (Drosophila) homolog
sonic hedgehog
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for resear |