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Anti-Cby1, clone 8-2
Species Reactivity Key Applications Host Format Antibody Type
M, H ICC, IP Mouse Purified Monoclonal Antibody
Description:
Anti-Cby1 Antibody, clone 8-2
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Specificity:
This antibody recognizes Cby1.
Molecular Weight:
~14 kDa
Epitope:
Unknown
Immunogen:
Recombinant protein corresponding to human Cby1.
Clone:
8-2
Isotype:
IgG2aκ
Background Information:
Chibby (Cby) is a β-catenin antagonist that is evolutionarily conserved from fly to human. Cby interacts with β-catenin in a manner that competes with TCF/LEF, and thus represses β-catenin-mediated gene activation. Cby and 14-3-3 protein form a trimolecular complex with β-catenin. Cby and 14-3-3 collaborate to promote cytoplasmic localization of β-catenin and thereby inhibit β-catenin signalling. Cby acts, in concert with 14-3-3 proteins, to facilitate nuclear export of β-catenin and thereby antagonize β-catenin signalling. Therefore, the inhibition of β-catenin signalling by Cby involves at least two distinct molecular mechanisms: competing with TCF/LEF transcription factors for β-catenin in the nucleus and facilitating nuclear export of β-catenin through interaction with 14-3-3 following its phosphorylation by AKT/PKB.
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Species Reactivity:
Mouse
Human
Species Reactivity Note:
Proven to react with human and mouse.
Application Notes:
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cby1 in NIH/3T3, A431, HeLa, and C2C12 cells.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Feng-Qian, L., et al. (2008). J. Cell Biol. 181(7):1141–1154.)
Western Blot Analysis: Western Blot is difficult to detect on endogenous lysates but has been shown to work in over expressed cell lines.
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Control:
NIH/3T3, A431, HeLa, and C2C12 cells
Quality Assurance:
Evaluated by Immunocytochemistry Analysis on NIH/3T3 cells.
Immunocytochemistry Analysis: This antibody positively stains the cytoplasm. The nucleus is also stained with less intensity.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9Y3M2
Entrez Gene Number:
NP_056188.1
Gene Symbol:
CBY1
ARB1
CBY
Cby
Chibby
PGEA1
PIGEA-14
PIGEA14
arb1
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Alternate Names:
ARPP-binding protein
Cytosolic leucine-rich protein
PKD2 interactor, Golgi and endoplasmic reticulum-associated 1
PKD2 interactor, golgi and endoplasmic reticulum associated 1
chibby homolog 1 (Drosophila)
chromosome 22 open reading frame 2
coiled-coil protein PIGEA-14 2
polycystin-2 interactor, Golgi- and endoplasmic reticulum-associated protein, 14 kDa
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Immunoprecipitation
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