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Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
Recommended Replacement for: Cat. # 05-953. 05-953 will only be availabe on a custom basis.
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REFERENCES
Cathepsin K expression and activity in canine osteosarcoma.
J M Schmit,H C Pondenis,A M Barger,L B Borst,L D Garrett,J M Wypij,Z L Neumann,T M Fan (0001) Journal of veterinary internal medicine / American College of Veterinary Internal Medicine.26
Species Reactivity Key Applications Host Format Antibody Type
Ca, H, M, Zebrafish WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
Replaces:
Cat. # 05-953. 05-953 will only be availabe on a custom basis.
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.
Molecular Weight:
55-60 kDa
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2
Modifications:
Phosphorylation
Clone:
A5S
Isotype:
IgG
Background Information:
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
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Species Reactivity:
Canine
Human
Mouse
Zebrafish
Species Reactivity Note:
: Predicted to cross-react with X. laevis based on sequence homology.
Control:
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Q15796
Entrez Gene Number:
NM_005901.4
NM_001003652.2
Gene Symbol:
SMAD2
hMAD-2
MGC22139
JV18
MADH2
MGC34440
hSMAD2
MADR2
JV18-1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcrip |