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Anti-Calmodulin Binding Protein Epitope Tag, clone C16T
Recommended Replacement for: 05-932
Species Reactivity Key Applications Host Format Antibody Type
Vrt WB Rabbit null Monoclonal Antibody
Description:
Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T | 04-932
Replaces:
05-932
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Specificity:
Recombinant proteins containing the calmodulin binding protein epitope tag.
Molecular Weight:
The molecular weight is related to the tagged protein.
Epitope:
CBP epitope
Immunogen:
KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.
Clone:
C16T
Isotype:
IgG
Background Information:
Calmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.
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Species Reactivity:
Vertebrates
Species Reactivity Note:
Vertebrates.
Control:
Samples containing the calmodulin binding protein epitope tag.
Quality Assurance:
Evaluated by Western Blot on lysates from transformed E. coli.
Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
Presentation:
Cultured supernatant with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P62158
Gene Symbol:
CALM
CALML2
CAM
CAM1
CAM2
CAM3
CAMB
CAMC
CAMI
CAMIII
CaM
DD132
PHKD
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Alternate Names:
calmodulin 1
calmodulin 1 (phosphorylase kinase, delta)
phosphorylase kinase, delta subunit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4
calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in |