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Anti-Histone H3, CT, pan, clone A3S Antibody (04-928)
Recommended Replacement for: 05-928
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REFERENCES
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Xiao, Tiaojiang, et al. (2003) Genes Dev., 17: 654-63 (2003)
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
Briggs, S D, et al. (2001) Genes Dev., 15: 3286-95 (2001)
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
Kizer, Kelby O, et al. (2005) Mol. Cell. Biol., 25: 3305-16 (2005)
Species Reactivity Key Applications Host Format Antibody Type
Ch, H, M, R, Yeast (S. cerevisiae) WB, ChIP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
Replaces:
05-928
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Trade Name:
Upstate (Millipore)
Product Overview:
An antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.
Specificity:
Recognizes Histone H3.
Molecular Weight:
17 kDa
Immunogen:
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3
Clone:
A3S
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Chicken
Human
Mouse
Rat
Yeast (S. cerevisiae)
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Species Reactivity Note:
Broad species cross-reactivity expected due to sequence homology
Application Notes:
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
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Quality Assurance:
Routinely evaluated by immunoblot analysis.
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
H3F3A
H3F3B
Alternate Names:
H3
Histone H3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any t |