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Anti-Smad2/3 Antibody, clone C4T
Recommended Replacement for: 05-914
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rabbit null Monoclonal Antibody
Description:
Anti-Smad2/3 Antibody, clone C4T | 04-914
Replaces:
05-914
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Specificity:
Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa.
Molecular Weight:
~50-60 kDa
Epitope:
a.a. 186-273
Immunogen:
Fusion protein corresponding to amino acids 186-273 of human Smad2.
Clone:
C4T
Background Information:
Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human. Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.
Control:
RIPA lysates from L6 cells
Quality Assurance:
Evaluated by Western Blot on L6 lysates.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Smad2/3 in L6 cell lysate.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q13485
Entrez Gene Number:
NP_001003652.1, NP_005893.1
Gene Symbol:
DPC4
JIP
MADH4
OTTHUMP00000163548
hSMAD4
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Alternate Names:
Deletion target in pancreatic carcinoma 4
MAD, mothers against decapentaplegic homolog 4
MAD, mothers against decapentaplegic homolog 4 (Drosophila)
Mothers against DPP homolog 4
SMAD 4
SMAD family member 4
SMAD, mothers against DPP homolog 4
SMAD, mothers against DPP homolog 4 (Drosophila)
deleted in pancreatic carcinoma locus 4
mothers against decapentaplegic homolog 4
mothers against decapentaplegic, Drosophila, homolog of, 4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans o |