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Anti-GSK3α/β
Recommended Replacement for: 05-903
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PRODUCT FAMILY INFORMATION
GSK-3 Antibodies, Proteins, Inhibitors
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways.
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. EMD Millipore’s GSK-3 antibodies, proteins and inhibitors are based on the expertise of Upstate, Chemicon, and Calbiochem. Our GSK-3 reagents have been well validated and published. See the ordering tab for a comprehensive list of our GSK-3 products and direct links to their technical pages for data, references and related products.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-GSK3α/β Antibody, rabbit monoclonal
Replaces:
05-903
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Specificity:
GSK3 alpha and beta.
Molecular Weight:
~46 kDa (beta) and 51 kDa (alpha)
Immunogen:
N-terminal 6His-tagged recombinant full length human GSK3β.
Isotype:
IgG
Background Information:
Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and mouse.
Predicted to cross-react with rat based on sequence homology.
Application Notes:
Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release® v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
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Control:
HEK293 cell lysate
Quality Assurance:
Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P49840
Entrez Gene Number:
NP_063937.2, NP_002084.2
Gene Symbol:
DKFZp686D0638
EC 2.7.11.26
Alternate Names:
GSK-3 alpha
glycogen synthase kinase 3 alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only a |