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Anti-Histone H4, pan Antibody (04-858)
Recommended Replacement for: 05-858
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REFERENCES
Replication-uncoupled histone deposition during adenovirus DNA replication.
Tetsuro Komatsu,Kyosuke Nagata (2012) Journal of virology.86
Multiple forms of H4 histone mRNA in human cells.
Lichtler, A C, et al. (1980) Proc. Natl. Acad. Sci. U.S.A., 77: 1942-6 (1980)
Species Reactivity Key Applications Host Format Antibody Type
Ch, H, Vrt, Yeast (S. cerevisiae) WB, Mplex, ChIP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Histone H4 Antibody, pan, rabbit monoclonal
Replaces:
05-858
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Trade Name:
Upstate (Millipore)
Product Overview:
This antibody recognizes histone H4 independant of any post-translational modifications.
Specificity:
Recognizes Histone H4.
Molecular Weight:
10 kDa
Epitope:
a.a. 25-28
Immunogen:
Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28
Clone:
62-141-13
Isotype:
IgG
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
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Species Reactivity:
Chicken
Human
Vertebrates
Yeast (S. cerevisiae)
Application Notes:
Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
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Control:
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
Quality Assurance:
Routinely evaluated by immunoblot using HeLa cell acid extract.
Presentation:
Cultured supernantant in 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P62805
Entrez Gene Number:
NM_003538.3
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
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