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Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
Recommended Replacement for: 05-857
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REFERENCES
Src protein-tyrosine kinase structure and regulation.
Roskoski, Robert (2004) Biochem. Biophys. Res. Commun., 324: 1155-64 (2004)
Molecular interdiction of Src-family kinase signaling in hematopoietic cells.
Geahlen, Robert L, et al. (2004) Oncogene, 23: 8024-32 (2004)
The interplay between Src family kinases and receptor tyrosine kinases.
Bromann, Paul A, et al. (2004) Oncogene, 23: 7957-68 (2004)
Structure and regulation of Src family kinases.
Boggon, Titus J and Eck, Michael J (2004) Oncogene, 23: 7918-27 (2004)
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Species Reactivity Key Applications Host Format Antibody Type
Vrt, M WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
Replaces:
05-857
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Trade Name:
Upstate (Millipore)
Specificity:
phospho-c-Src (Tyr416)
Molecular Weight:
60kDa
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src
Modifications:
Phosphorylation
Clone:
2N8
Isotype:
IgG
Background Information:
Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
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Species Reactivity:
Vertebrates
Mouse
Species Reactivity Note:
Mouse. Broad species cross-reactivity expected based on sequence homology.
Application Notes:
Western Blot Analysis:
A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).
Immunoprecipitation:
A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).
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Control:
RIPA cell lysate
Quality Assurance:
Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186)
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P12931
Entrez Gene Number:
NM_005417
Gene Symbol:
pp60c-src
ASV
c-SRC
SRC1
p60-Src
c-src
SRC
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Alternate Names:
KAT13A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used f |