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Anti-phospho-STAT5A/B (Tyr694/699), clone A11W
Recommended Replacement for: 05-886
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REFERENCES
H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling.
John F Woolley,Ruth Naughton,Joanna Stanicka,David R Gough,Lavinia Bhatt,Bryan C Dickinson,Christopher J Chang,Thomas G Cotter (2012) PloS one.7
Altered interleukin-12 responsiveness in Th1 and Th2 cells is associated with the differential activation of STAT5 and STAT1.
Gollob, J A, et al. (1998) Blood, 91: 1341-54 (1998)
Species Reactivity Key Applications Host Format Antibody Type
R, H, M, B, Chp WB, not IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal
Replaces:
05-886
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Specificity:
Phospho-STAT5A/B (Tyr694/699).
Molecular Weight:
~95 kDa
Epitope:
pTyr694/699
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.
Modifications:
Phosphorylation
Clone:
A11W
Isotype:
IgG
Background Information:
The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.
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Species Reactivity:
Rat
Human
Mouse
Bovine
Chimpanzee
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Applications Not Recommended:
Immunoprecipitation
Control:
PDGF treated 3T3/NIH cells
Quality Assurance:
Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.
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Presentation:
Cultured supernatant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P42229
Entrez Gene Number:
NM_003152 (STAT5A), NM_012448 (STAT5B)
Gene Symbol:
MGF
STAT5
STAT5
Alternate Names:
signal transducer and activator of transcription 5A
signal transducer and activator of transcription 5B
transcription factor STAT5B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth fac |