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Anti-TBK1, clone AOW9
Recommended Replacement for: 05-856
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REFERENCES
Triggering the interferon antiviral response through an IKK-related pathway.
Sharma, Sonia, et al. (2003) Science, 300: 1148-51 (2003)
IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
Fitzgerald, Katherine A, et al. (2003) Nat. Immunol., 4: 491-6 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, M, Ca, Ch WB, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal
Replaces:
05-856
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Specificity:
TBK1; Does not cross-react with IKKε, IKKα or IKKβ.
Molecular Weight:
85 kDa
Epitope:
a.a. 642-645
Immunogen:
Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.
Clone:
AOW9
Isotype:
IgG
Background Information:
TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.
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Species Reactivity:
Human
Mouse
Canine
Chicken
Species Reactivity Note:
Human. Predicted to cross-react with mouse, dog, and chicken based on sequence homology.
Application Notes:
Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
Control:
RIPA lysates from HEK293 cells.
Quality Assurance:
Evaluated by western blot on RIPA lysates from HEK293 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
Presentation:
Cultured supernatant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q9UHD2
Entrez Gene Number:
NP_037386
Gene Symbol:
EC 2.7.11.1
FLJ11330
NAK
T2K
Alternate Names:
NF-kB-activating kinase
NF-kappa-B-activating kinase
TANK-binding kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
The NF-k |