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Anti-Estrogen Receptor β, clone 68-4 Antibody
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REFERENCES
Abnormal vascular function and hypertension in mice deficient in estrogen receptor beta.
Zhu, Yan, et al. (2002) Science, 295: 505-8 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
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Specificity:
Estrogen receptor β, long form, does not detect estrogen receptor alpha.
Molecular Weight:
59kDa
Epitope:
a.a.55-74(H) or 63-82 (R, M)
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β.
Clone:
68-4
Isotype:
IgG
Background Information:
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Reacts with human. Predicted to cross-react with mouse and rat based on sequence homology.
Application Notes:
Immunoprecipitation:
10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate.
Control:
MCF7 cell extracts
Quality Assurance:
Evaluated by western blot on RIPA lysates from MCF-7 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells.
Presentation:
Cultured supernatant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P03372
Entrez Gene Number:
NM_001437
Gene Symbol:
DKFZp686N23123
ER
ER-alpha
ESR
ESRA
Era
NR3A1
OTTHUMP00000017718
OTTHUMP00000017719
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Alternate Names:
Estradiol receptor
Nuclear receptor subfamily
estrogen receptor 1
estrogen receptor alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to hum |