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Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal
Recommended Replacement for: 05-825
Species Reactivity Key Applications Host Format Antibody Type
M, H IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal
Replaces:
05-825
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Trade Name:
Upstate (Millipore)
Specificity:
PDGFRβ, does not cross-react with EGF receptor
Molecular Weight:
190kDa
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β.
Clone:
4A56
Isotype:
IgG
Species Reactivity:
Mouse
Human
Species Reactivity Note:
Mouse and human.
Application Notes:
Westerm Blot Analysis:
A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate.
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Control:
RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P09619
Entrez Gene Number:
NM_002608
Gene Symbol:
PDGF-R-beta
CD140B
JTK12
PDGFR
CD140b
PDGFR1
PDGFRB
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
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UniProt Summary:
FUNCTION: SwissProt: P09619 # Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
SIZE: 1106 amino acids; 123968 Da
SUBUNIT: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly.
SUBCELLULAR LOCATION: Membrane; |