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Anti-phospho-Histone H3 (Ser10), clone MC463; 100 μL
Recommended Replacement for: 05-817
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REFERENCES
Akt2 interacts with Snail1 in the E-cadherin promoter.
Villagrasa, P, et al. (2011) Oncogene, (2011)
K+ efflux is required for histone H3 dephosphorylation by Listeria monocytogenes listeriolysin O and other pore-forming toxins.
Hamon MA, Cossart P (2011) Infection and immunity.79:2839-46. Epub 2011 Apr 11.
In vitro profiling of epigenetic modifications underlying heavy metal toxicity of tungsten-alloy and its components.
Ranjana Verma,Xiufen Xu,Manoj K Jaiswal,Cara Olsen,David Mears,Giuseppina Caretti,Zygmunt Galdzicki (2011) Toxicology and applied pharmacology.253
Notch- and transducin-like enhancer of split (TLE)-dependent histone deacetylation explain interleukin 12 (IL-12) p70 inhibition by zymosan.
Yolanda Alvarez,Cristina Municio,Etzel Hugo,Jimmy Zhu,Sara Alonso,Xiaoyu Hu,Nieves Fernández,Mariano Sánchez Crespo (2011) The Journal of biological chemistry.286
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Species Reactivity Key Applications Host Format Antibody Type
H IF, WB, ICC, ChIP-seq, DB, Mplex, ChIP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
Replaces:
05-817
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Trade Name:
Upstate (Millipore)
Specificity:
Histone H3 phosphorylated at Serine 10
Molecular Weight:
17kDa
Immunogen:
Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3
Modifications:
Phosphorylation
Clone:
MC463
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine
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Species Reactivity:
Human
Species Reactivity Note:
Broad species cross-reactivity is expected
Application Notes:
Western Blot Analysis:
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).
Beadlyte® Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.
Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.
Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.
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Control:
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
Quality Assurance:
routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells
Presentation:
Cultured supernantant in 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
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