|
Anti-phospho-PPARγ (Ser82) Antibody, clone AW504
Recommended Replacement for: 05-816
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rabbit null Monoclonal Antibody
Description:
Anti-phospho-PPARγ (Ser82) Antibody, clone AW504 | 04-816
Replaces:
05-816
Promotional Text:
Special Offer on Antibodies! Click Here!
Specificity:
PPARγ phosphorylated at Ser82, Mr 55 & 60 kDa.
Molecular Weight:
~55 & 60 kDa
Epitope:
pSer82
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence PApSPP in which pS corresponds to phosphoserine at residue 82 of human PPARγ.
Modifications:
Phosphorylation
Clone:
AW504
Isotype:
IgG
Background Information:
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: , and . PPAR stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs can bind to DNA only as a heterodimer with the retinoid X receptor (RXR).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and mouse. Predicted to cross-react with rat based on sequence homology.
Control:
3T3/L1 cells differentiated into adipocytes, PMA-stimulated THP-1 cells.
Quality Assurance:
Evaluated by western blot on NIH/3T3 L1 cells that were undifferentiated and differentiated into adipocytes.
Western Blot Analysis: 1:500 dilution of this antibody detected PPARγ phosphorylated at Ser82 in undifferentiated and acipocyte differentiated NIH/3T3 L1 cells.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P37231
Entrez Gene Number:
NP_005028.4
Gene Symbol:
CIMT1
NR1C3
OTTHUMP00000185030
OTTHUMP00000185033
OTTHUMP00000185037
PPAR-gamma
PPARG1
PPARG2
View All »
Alternate Names:
Nuclear receptor subfamily 1 group C member 3
PPAR gamma
peroxisome proliferative activated receptor gamma
peroxisome proliferator-activated receptor gamma
peroxisome proliferator-activated receptor gamma 1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of ad |