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Anti-Estrogen Receptor α, clone 60C Antibody
Recommended Replacement for: 04-820 is a recommended replacement for 05-820
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REFERENCES
Activation of rapid oestrogen signalling in aggressive human breast cancers.
Poulard, Coralie, et al. (2012) EMBO Mol Med, (2012)
Activation function 2 (AF2) of estrogen receptor-alpha is required for the atheroprotective action of estradiol but not to accelerate endothelial healing.
Audrey Billon-Galés,Andrée Krust,Coralie Fontaine,Anne Abot,Gilles Flouriot,Céline Toutain,Hortense Berges,Alain-Pierre Gadeau,Françoise Lenfant,Pierre Gourdy,Pierre Chambon,Jean-François Arnal (2011) Proceedings of the National Academy of Sciences of the United States of America.108
ATBF1 inhibits estrogen receptor (ER) function by selectively competing with AIB1 for binding to the ER in ER-positive breast cancer cells.
Dong, Xue-Yuan, et al. (2010) J. Biol. Chem., 285: 32801-9 (2010)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, B, Po WB, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
Replaces:
04-820 is a recommended replacement for 05-820
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Specificity:
Recognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β.
Molecular Weight:
66 kDa
Epitope:
N-term
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine.
Clone:
60C
Isotype:
IgG
Background Information:
The Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.
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Species Reactivity:
Human
Mouse
Rat
Bovine
Pig
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Species Reactivity Note:
Human. Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology.
Application Notes:
Immunoprecipitation:
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.
Quality Assurance:
Routinely evaluated by western blot analysis.
Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
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Purification Method:
Unpurified
Presentation:
Cultured supernantant with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P03372
Entrez Gene Number:
NP_000116.2
Gene Symbol:
ER
ER-alpha
ESR
ESRA
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