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Anti-phospho-eNOS/NOS III (Thr495)
Recommended Replacement for: Cat. # 05-811. 05-811 will only be availabe on a custom basis.
Species Reactivity Key Applications Host Format Antibody Type
B WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal
Replaces:
Cat. # 05-811. 05-811 will only be availabe on a custom basis.
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Trade Name:
Upstate (Millipore)
Product Overview:
Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Specificity:
Recognizes phosphorylated eNOS/NOS III.
Molecular Weight:
135 kDa
Immunogen:
Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Bovine
Species Reactivity Note:
Predicted cross-reactivity with human, rabbit, mouse and rat
Application Notes:
Western Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
Control:
Fetal bovine heart endothelial cell lysate
Quality Assurance:
routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P29474
Entrez Gene Number:
NM_000603
Gene Symbol:
NOS3
cNOS
ECNOS
eNOS
EC-NOS
NOSIII
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Alternate Names:
NOS III
endothelial nitric oxidase synthase
endothelial nitric oxide synthase
nitric oxide synthase 3 (endothelial cell)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
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Brand Family:
Upstate
Product Name:
Anti-phosph |