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Anti-SET7/9 Antibody, clone 5F2.3
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-SET7/9 Antibody, clone 5F2.3
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes human SET7/9
Molecular Weight:
49 kDa
Epitope:
N-terminal region
Immunogen:
N-terminal His-tagged human SET9 recombinant protein
Clone:
5F2.3
Background Information:
The methylation of histones plays a pivotal role in the regulation of chromatin structure and gene expression. Histone methylation can occur on Arg or Lys residues, with an exquisite site selectivity for Lys methylation at specific positions in the N-termini of histones H3 and H4. SET7/9, a histone methyltransferase (HMTase), which transfers methyl groups to Lys4 of Histone H3, forms a complex with S-adenosyl-L-methionine. This complex contains an active site consisting of a binding pocket where an AdoMet molecule, in an unusual conformation, binds a narrow substrate-specific channel that only unmethylated lysine residues can access and a catalytic tyrosine residue.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Not yet tested in other species.
Quality Assurance:
Routinely evaluated by western blot on RIPA lysates from NIH/3T3 cells.
Presentation:
100 μg of protein G purified mouse IgG1κ in Tris-Glycine with 0.05% NaN3.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
UniProt Number:
Q8WTS6
Entrez Gene Number:
AAL69901
Alternate Names:
SetD7
Histone-lysine N-methyltransferase SETD7
EC 2.1.1.43
SET domain-containing protein 7
SET7/9
Histone H3-K4 methyltransferase SETD7
Set7
Set9
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
SUBUNIT: Interacts with IPF1/PDX-1.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Widely expressed. Expressed in pancreatic islets.
DOMAIN: The SET domain is necessary but not sufficient for histone methyltransferase activity.
MISCELLANEOUS: Monomethyltransferase activity is achieved by disrupting the formation at near-attack conformations for the dimethylation reaction.
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Brand Family:
Upstat |