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Anti-NFAT1, clone 8G4.2
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IF Mouse Purified Monoclonal Antibody
Description:
Anti-NFAT1 Antibody, clone 8G4.2
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Trade Name:
Upstate (Millipore)
Specificity:
Detects NFAT1.
Molecular Weight:
120-140 kDa
Epitope:
N-term
Immunogen:
GST-fusion protein corresponding to amino acids 1-297 of human NFAT1.
Clone:
8G4.2
Isotype:
IgMκ
Background Information:
NFAT (Nuclear Factor of Activated T-cells) is a family of transcription factors that are located in the cytoplasm in their inactive state. NFAT translocates to the nucleus upon stimulation of the T-cell receptor where it plays a key role in gene expression in immune response. NFAT is dephosphorylated and activated by the phosphatase PP2B/Calcineurin. This results in its nuclear localization and transcriptional activity. For NFAT1 (NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), calcineurin is known to target the region between residues 114-126. Mutations in this region of NFAT1 result in its inability to de-phosphorylate and subsequent nuclear translocation. Upon activation, NFAT mediates the transcriptional induction of cytokines and other immuno-regulatory genes during immune responses, and is expressed in T-cells, B-cells, mast cells and NK cells.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, and Rat. Other species have not been tested.
Quality Assurance:
Routinely evaluated by Western Blot
Purification Method:
Ammonium sulfate precipitation
Presentation:
Purified mouse monoclonal in PBS with 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
Q13469
Entrez Gene Number:
NP_001129493.1
Gene Symbol:
KIAA0611
NF-ATP
NF-ATp
NFAT1
NFATP
NFATp
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Alternate Names:
NFAT pre-existing subunit
NFAT transcription complex, preexisting component
T cell transcription factor NFAT1
nuclear factor of activated T-cells, cytoplasmic
nuclear factor of activated T-cells, cytoplasmic,
calcineurin-dependent 2
nuclear factor of activated T-cells, preexisting component 2
preexisting nuclear factor of activated T-cells 2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunofluorescence
Entrez Gene Summary:
This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding differen |