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Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody
Recommended Replacement for: 05-808
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REFERENCES
Histone H3 Arg2 methylation provides alternative directions for COMPASS.
Klose, Robert J and Zhang, Yi (2007) Nat. Struct. Mol. Biol., 14: 1058-60 (2007)
Histone H3K4 demethylases are essential in development and differentiation.
Benevolenskaya, Elizaveta V (2007) Biochem. Cell Biol., 85: 435-43 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt DB, Mplex, PIA, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal
Replaces:
05-808
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes dimethyl-arginine at residue 2 of histone H3, Mr 17kDa. Additional unidentified bands above 50 kDa are detected in some samples.
Molecular Weight:
17kDa
Epitope:
Arg2 of Histone H3
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …Ame2RTKQ…, in which me2R corresponds to dimethyl-arginine 2 of human histone H3.
Modifications:
Methylation
Clone:
20.2
Isotype:
IgG
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 Arg2 methylation is a widespread silencing modification that inhibits histone H3 Lys4 trimethylation.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. The immunizing sequence is highly conserved evolutionarily, so broad cross-reactivity is expected.
Application Notes:
Dot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 04-808, Anti-dimethyl H3 (Arg2) at 1ug/mL (1:1000) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Peptide Inhibition Analysis:
2 μM of histone H3 peptide containing dimethyl-Arg2 abolished detection of histone H3 by a previous lot of anti-dimethyl-Histone H3(Arg2) (1:4,000 dilution) in immunoblots of HeLa acid extracts. Some competition of signal was also seen with unmodified peptide containing Arg2.
Peptide Dot Blot Analysis:
A 1:4,000-1:16,000 dilution of a previous lot only detected histone H3 peptide containing dimethyl-Arg2. Peptides containing unmodi-fied Arg2, dimethyl-Arg17 or dimethyl-Arg26 were not detected.
Beadlyte® Histone Peptide Assay:
1:500-1:48,000 dilutions of a previous lot detected histone H3 peptide containing dimethyl-Arg2. Cross-reactivity to peptides containing unmodified Arg2 or dimethylArg 17 or 26 was not seen.
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